Genomic insights into the evolution and mechanisms of carbapenem-resistant hypervirulent Klebsiella pneumoniae co-harboring bla_KPC and bla_NDM: implications for public health threat mitigation.

ABSTRACT:

Background

Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) co-producing bla_KPC and bla_NDM poses a serious threat to public health. This study aimed to investigate the mechanisms underlying the resistance and virulence of CR-hvKP isolates collected from a Chinese hospital, with a focus on bla_KPC and bla_NDM dual-positive hvKP strains.

Methods

Five CR-hvKP strains were isolated from a teaching hospital in China. Antimicrobial susceptibility and plasmid stability testing, plasmid conjugation, pulsed-field gel electrophoresis, and whole-genome sequencing (WGS) were performed to examine the mechanisms of resistance and virulence. The virulence of CR-hvKP was evaluated through serum-killing assay and Galleria mellonella

SUBMITTER: Wang Q 

PROVIDER: S-EPMC10981300 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature