DEAD box RNA helicase 5 is a new pro-viral host factor for Sindbis virus infection.

ABSTRACT:

Background

RNA helicases are emerging as key factors regulating host-virus interactions. The DEAD-box ATP-dependent RNA helicase DDX5, which plays an important role in many aspects of cellular RNA biology, was also found to either promote or inhibit viral replication upon infection with several RNA viruses. Here, our aim is to examine the impact of DDX5 on Sindbis virus (SINV) infection.

Methods

We analysed the interaction between DDX5 and the viral RNA using imaging and RNA-immunoprecipitation approaches. The interactome of DDX5 in mock- and SINV-infected cells was determined by mass spectrometry. We validated the interaction between DDX17 and the viral capsid by co- immunoprecipitation in the presence or absence of an RNase treatment. We determined the subcellular localiza

SUBMITTER: Messmer M 

PROVIDER: S-EPMC10981342 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature