IL-6-Derived Autocrine Lactate Promotes Immune Escape of Uveal Melanoma.

ABSTRACT:

Purpose

Early metastasis, in which immune escape plays a crucial role, is the leading cause of death in patients with uveal melanoma (UM); however, the molecular mechanism underlying UM immune escape remains unclear, which greatly limits the clinical application of immunotherapy for metastatic UM.

Methods

Transcriptome profiles were revealed by RNA-seq analysis. TALL-104 and NK-92MI-mediated cell killing assays were used to examine the immune resistance of UM cells. The glycolysis rate was measured by extracellular acidification analysis. Protein stability was evaluated by CHX-chase assay. Immunofluorescence histochemistry was performed to detect protein levels in clinical UM specimens.

Results

Continuous exposure to IL-6 induced the expression of both PD-L1 and HLA-E

SUBMITTER: Gong C 

PROVIDER: S-EPMC10981435 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature