A lineage-resolved cartography of microRNA promoter activity in C. elegans empowers multidimensional developmental analysis.

ABSTRACT: Elucidating the expression of microRNAs in developing single cells is critical for functional discovery. Here, we construct scCAMERA (single-cell cartography of microRNA expression based on reporter assay), utilizing promoter-driven fluorescent reporters in conjunction with imaging and lineage tracing. The cartography delineates the transcriptional activity of 54 conserved microRNAs in lineage-resolved single cells throughout C. elegans embryogenesis. The combinatorial expression of microRNAs partitions cells into fine clusters reflecting their function and anatomy. Notably, the expression of individual microRNAs exhibits high cell specificity and divergence among family members. Guided by cellular expression patterns, we identify developmental functions of specific microRNAs, including mi

SUBMITTER: Xu W 

PROVIDER: S-EPMC10981687 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature