FOXO1 reshapes neutrophils to aggravate acute brain damage and promote late depression after traumatic brain injury.

ABSTRACT:

Background

Neutrophils are traditionally viewed as first responders but have a short onset of action in response to traumatic brain injury (TBI). However, the heterogeneity, multifunctionality, and time-dependent modulation of brain damage and outcome mediated by neutrophils after TBI remain poorly understood.

Methods

Using the combined single-cell transcriptomics, metabolomics, and proteomics analysis from TBI patients and the TBI mouse model, we investigate a novel neutrophil phenotype and its associated effects on TBI outcome by neurological deficit scoring and behavioral tests. We also characterized the underlying mechanisms both in vitro and in vivo through molecular simulations, signaling detections, gene expression regulation assessments [including dual-luciferase rep

SUBMITTER: Zhou M 

PROVIDER: S-EPMC10981823 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature