Subpopulation commensalism promotes Rac1-dependent invasion of single cells via laminin-332.

ABSTRACT: Phenotypic heterogeneity poses a significant hurdle for cancer treatment but is under-characterized in the context of tumor invasion. Amidst the range of phenotypic heterogeneity across solid tumor types, collectively invading cells and single cells have been extensively characterized as independent modes of invasion, but their intercellular interactions have rarely been explored. Here, we isolated collectively invading cells and single cells from the heterogeneous 4T1 cell line and observed extensive transcriptional and epigenetic diversity across these subpopulations. By integrating these datasets, we identified laminin-332 as a protein complex exclusively secreted by collectively invading cells. Live-cell imaging revealed that laminin-332 derived from collectively invading cells increas

SUBMITTER: Yoon SB 

PROVIDER: S-EPMC10982113 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature