Immune cell phenotype and function patterns across the life course in individuals from rural Uganda.

ABSTRACT:

Background

To determine the pattern of immune cell subsets across the life span in rural sub-Saharan Africa (SSA), and to set a reference standard for cell subsets amongst Africans, we characterised the major immune cell subsets in peripheral blood including T cells, B cells, monocytes, NK cells, neutrophils and eosinophils, in individuals aged 3 to 89 years from Uganda.

Methods

Immune phenotypes were measured using both conventional flow cytometry in 72 individuals, and full spectrum flow cytometry in 80 individuals. Epstein-Barr virus (EBV) IFN-γ T cell responses were quantified in 332 individuals using an ELISpot assay. Full blood counts of all study participants were also obtained.

Results

The percentages of central memory (T_CM) and senescent CD4+ and C

SUBMITTER: Nalwoga A 

PROVIDER: S-EPMC10982424 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature