Transcriptional and translational landscape fine-tune genome annotation and explores translation control in cotton.

ABSTRACT:

Introduction

The unavailability of intergenic region annotation in whole genome sequencing and pan-genomics hinders efforts to enhance crop improvement.

Objectives

Despite advances in research, the impact of post-transcriptional regulation on fiber development and translatome profiling at different stages of fiber growth in cotton (G. hirsutum) remains unexplored.

Methods

We utilized a combination of reference-guided de novo transcriptome assembly and ribosome profiling techniques to uncover the hidden mechanisms of translational control in eight distinct tissues of upland cotton.

Results

Our study identified P-site distribution at three-nucleotide periodicity and dominant ribosome footprint at 27 nucleotides. Specifically, we have detected 1,589 small open rea

SUBMITTER: Qanmber G 

PROVIDER: S-EPMC10982868 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature