Dataset Information

Effects of Drag-Reducing Polymers on Hemodynamics and Whole Blood-Endothelial Interactions in 3D-Printed Vascular Topologies.

ABSTRACT: Most in vitro models use culture medium to apply fluid shear stress to endothelial cells, which does not capture the interaction between blood and endothelial cells. Here, we describe a new system to characterize whole blood flow through a 3D-printed, endothelialized vascular topology that induces flow separation at a bifurcation. Drag-reducing polymers, which have been previously studied as a potential therapy to reduce the pressure drop across the vascular bed, are evaluated for their effect on mitigating the disturbed flow. Polymer concentrations of 1000 ppm prevented recirculation and disturbed flow at the wall. Proteomic analysis of plasma collected from whole blood recirculated through the vascularized channel with and without drag-reducing polymers provides insight into the effects of flow regimes on levels of proteins indicative of the endothelial-blood interaction. The results indicate that blood flow alters proteins associated with coagulation, inflammation, and other processes. Overall, these proof-of-concept experiments demonstrate the importance of using whole blood flow to study the endothelial response to perfusion.

SUBMITTER: Paone LS

PROVIDER: S-EPMC10982934 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature

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Effects of Drag-Reducing Polymers on Hemodynamics and Whole Blood-Endothelial Interactions in 3D-Printed Vascular Topologies.

Paone Louis S LS Szkolnicki Matthew M DeOre Brandon J BJ Tran Kiet A KA Goldman Noah N Andrews Allison M AM Ramirez Servio H SH Galie Peter A PA

ACS applied materials & interfaces 20240315 12

Most in vitro models use culture medium to apply fluid shear stress to endothelial cells, which does not capture the interaction between blood and endothelial cells. Here, we describe a new system to characterize whole blood flow through a 3D-printed, endothelialized vascular topology that induces flow separation at a bifurcation. Drag-reducing polymers, which have been previously studied as a potential therapy to reduce the pressure drop across the vascular bed, are evaluated for their effect o ...[more]

PMID: 38488736

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Project description:IntroductionSurgery, a crucial therapeutic modality in the treatment of solid tumors, can induce sterile inflammatory processes which can result in metastatic progression. Liver ischemia and reperfusion (I/R) injury, an inevitable consequence of hepatic resection of metastases, has been shown to foster hepatic capture of circulating cancer cells and accelerate metastatic growth. Efforts to reduce these negative consequences have not been thoroughly investigated. Drag reducing polymers (DRPs) are blood-soluble macromolecules that can, in nanomolar concentrations, increase tissue perfusion, decrease vascular resistance and decrease near-wall microvascular concentration of neutrophils and platelets thereby possibly reducing the inflammatory microenvironment. We hypothesize that DRP can potentially be used to ameliorate metastatic capture of tumor cells and tumor growth within the I/R liver.MethodsExperiments were performed utilizing a segmental ischemia model of mice livers. Five days prior or immediately prior to ischemia, murine colon adenocarcinoma cells (MC38) were injected into the spleen. DRP (polyethylene oxide) or a control of low-molecular-weight polyethylene glycol without drag reducing properties were administered intraperitoneally at the onset of reperfusion.ResultsAfter three weeks from I/R, we observed that liver I/R resulted in an increased ability to capture and foster growth of circulating tumor cells; in addition, the growth of pre-existing micrometastases was accelerated three weeks later. These effects were significantly curtailed when mice were treated with DRPs at the time of I/R. Mechanistic investigations in vivo indicated that DRPs protected the livers from I/R injury as evidenced by significant decreases in hepatocellular damage, neutrophil recruitment into the liver, formation of neutrophil extracellular traps, deposition of platelets, formation of microthrombi within the liver sinusoids and release of inflammatory cytokines.ConclusionsDRPs significantly attenuated metastatic tumor development and growth. DRPs warrant further investigation as a potential treatment for liver I/R injury in the clinical setting to improve cancer-specific outcomes.

Project description:Frictional pressure drop has been grasping the attention of many industrial applications associated with multi-phase and academia. Alongside the United Nations, the 2030 Agenda for Sustainable Development calls for the exigency of giving attention to economic growth, a considerable reduction in power consumption is necessary to co-up with this vision and to adhere to energy-efficient practices. Thereinto, drag-reducing polymers (DRPs), which do not require additional infrastructure, are a much better option for increasing energy efficiency in a series of critical industrial applications. Therefore, this study evaluates the effects of two DRPs-polar water-soluble polyacrylamide (DRP-WS) and nonpolar oil-soluble polyisobutylene (DRP-OS)-on energy efficiency for single-phase water and oil flows, two-phase air-water and air-oil flows, and three-phase air-oil-water flow. The experiments were conducted using two different pipelines; horizontal polyvinyl chloride with an inner diameter of 22.5 mm and horizontal stainless steel with a 10.16 mm internal diameter. The energy-efficiency metrics are performed by investigating the head loss, percentage saving in energy consumption (both per unit pipe length), and throughput improvement percentage (%TI). The larger pipe diameter was used in experiments for both DRPs, and it was discovered that despite the type of flow or variations in liquid and air flow rates, there was a reduction in head loss, an increase in energy savings, and an increase in the throughput improvement percentage. In particular, DRP-WS is found to be more promising as an energy saver and the consequent savings in the infrastructure cost. Hence, equivalent experiments of DRP-WS in two-phase air-water flow using a smaller pipe diameter show that the head loss drastically increases. However, the percentage saving in power consumption and throughput improvement percentage is significantly compared with that found in the larger pipe. Thus, this study found that DRPs can improve energy efficiency in various industrial applications, with DRP-WS being particularly promising as an energy saver. However, the effectiveness of these polymers may vary depending on the flow type and pipe diameter.

Dataset Information

Effects of Drag-Reducing Polymers on Hemodynamics and Whole Blood-Endothelial Interactions in 3D-Printed Vascular Topologies.

Publications

Effects of Drag-Reducing Polymers on Hemodynamics and Whole Blood-Endothelial Interactions in 3D-Printed Vascular Topologies.

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