Arginine-Selective Bioconjugation Reagent for Effective ¹⁸F-labeling of Native Proteins.

ABSTRACT: Protein-based ¹⁸F-PET tracers offer new possibilities in early disease detection and personalized medicine. Their development relies heavily on the availability and effectiveness of ¹⁸F-prosthetic groups. We prepared and evaluated a novel arginine-selective prosthetic group, 4-[¹⁸F]fluorophenylglyoxal ([¹⁸F]FPG). [¹⁸F]FPG was radiosynthesized by a one-pot, two-step procedure with a non-decay-corrected (n.d.c.) isolated radiochemical yield (RCY) of 41 ± 8% (n = 10). [¹⁸F]FPG constitutes a generic tool for ¹⁸F-labeling of various proteins, including human serum albumin (HSA), ubiquitin, interleukin-2, and interleukin-4 in ∼30-60% n.d.c. isolated RCYs. [¹⁸F]FPG conjugation with arginine residues is

SUBMITTER: Sadasivam P 

PROVIDER: S-EPMC10982996 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature