Role of Galectin-3 in intervertebral disc degeneration: an experimental study.

ABSTRACT:

Background

This study investigated the role of Galectin-3 in the degeneration of intervertebral disc cartilage.

Methods

The patients who underwent lumbar spine surgery due to degenerative disc disease were recruited and divided into Modic I, Modic II, and Modic III; groups. HE staining was used to detect the pathological changes in endplates. The changes of Galectin-3, MMP3, Aggrecan, CCL3, and Col II were detected by immunohistochemistry, RT-PCR, and Western blot. MTT and flow cytometry were used to detect cartilage endplate cell proliferation, cell cycle, and apoptosis.

Results

With the progression of degeneration (from Modic I to III), the chondrocytes and density of the cartilage endplate of the intervertebral disc decreased, and the collagen arrangement of the ca

SUBMITTER: Li J 

PROVIDER: S-EPMC10983641 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature