Transcriptomics aids in uncovering the metabolic shifts and molecular machinery of Schizochytrium limacinum during biotransformation of hydrophobic substrates to docosahexaenoic acid.

ABSTRACT:

Background

Biotransformation of waste oil into value-added nutraceuticals provides a sustainable strategy. Thraustochytrids are heterotrophic marine protists and promising producers of omega (ω) fatty acids. Although the metabolic routes for the assimilation of hydrophilic carbon substrates such as glucose are known for these microbes, the mechanisms employed for the conversion of hydrophobic substrates are not well established. Here, thraustochytrid Schizochytrium limacinum SR21 was investigated for its ability to convert oils (commercial oils with varying fatty acid composition and waste cooking oil) into ω-3 fatty acid; docosahexaenoic acid (DHA).

Results

Within 72 h SR21 consumed ~ 90% of the oils resulting in enhanced biomass (7.5 g L^- 1) which was 2-fold hig

SUBMITTER: Mariam I 

PROVIDER: S-EPMC10983653 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature