Sialylation on vesicular integrin β1 determined endocytic entry of small extracellular vesicles into recipient cells.

ABSTRACT:

Background

Small extracellular vesicles (sEV) are closely associated with the development and metastasis of many types of mammalian cancer. Glycoconjugates are highly expressed on sEV and play important roles in sEV biogenesis and their interaction with other cells. However, the study on vesicular glycoconjugates are far behind proteins and nucleic acids. Especially, the functions of sialic acids which are the terminal components of glycoconjugates, are poorly understood in sEV.

Methods

Sialic acid levels on sEV from plasma and bladder cancer cells were determined by ELISA and lectin blotting. Effects of sialylation on sEV uptake were determined by flow cytometry. Vesicular glycoproteins bearing sialic acids responsible for sEV uptake was identified by proteomics and density

SUBMITTER: Lin M 

PROVIDER: S-EPMC10983696 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature