NAT10-mediated ac⁴C modification promotes ectoderm differentiation of human embryonic stem cells via acetylating NR2F1 mRNA.

ABSTRACT: Cell fate determination in mammalian development is complex and precisely controlled and accumulating evidence indicates that epigenetic mechanisms are crucially involved. N⁴-acetylcytidine (ac⁴C) is a recently identified modification of messenger RNA (mRNA); however, its functions are still elusive in mammalian. Here, we show that N-acetyltransferase 10 (NAT10)-mediated ac⁴C modification promotes ectoderm differentiation of human embryonic stem cells (hESCs) by acetylating nuclear receptor subfamily 2 group F member 1 (NR2F1) mRNA to enhance translation efficiency (TE). Acetylated RNA immunoprecipitation sequencing (acRIP-seq) revealed that levels of ac⁴C modification were higher in ectodermal neuroepithelial progenitor (NEP) cells than in hESCs

SUBMITTER: Ge J 

PROVIDER: S-EPMC10984107 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature