Drug repurposing screening and mechanism analysis based on human colorectal cancer organoids.

ABSTRACT: Colorectal cancer (CRC) is a highly heterogeneous cancer and exploring novel therapeutic options is a pressing issue that needs to be addressed. Here, we established human CRC tumor-derived organoids that well represent both morphological and molecular heterogeneities of original tumors. To efficiently identify repurposed drugs for CRC, we developed a robust organoid-based drug screening system. By combining the repurposed drug library and computation-based drug prediction, 335 drugs were tested and 34 drugs with anti-CRC effects were identified. More importantly, we conducted a detailed transcriptome analysis of drug responses and divided the drug response signatures into five representative patterns: differentiation induction, growth inhibition, metabolism inhibition, immune response pro

SUBMITTER: Mao Y 

PROVIDER: S-EPMC10984622 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature