Overexpression of HMGB1 in hepatocytes accelerates PTEN inactivation-induced liver cancer.

ABSTRACT:

Background

Liver cancer is increasing due to the rise in metabolic dysfunction-associated steatohepatitis (MASH). High-mobility group box-1 (HMGB1) is involved in the pathogenesis of chronic liver disease, but its role in MASH-associated liver cancer is unknown. We hypothesized that an increase in hepatocyte-derived HMGB1 in a mouse model of inactivation of PTEN that causes MASH could promote MASH-induced tumorigenesis.

Methods

We analyzed publicly available transcriptomics datasets, and to explore the effect of overexpressing HMGB1 in cancer progression, we injected 1.5-month-old Pten∆Hep mice with adeno-associated virus serotype-8 (AAV8) vectors to overexpress HMGB1-EGFP or EGFP, and sacrificed them at 3, 9 and 11 months of age.

Results

We found that HMGB1 mRNA incr

SUBMITTER: Athavale D 

PROVIDER: S-EPMC10984663 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature