The P124A mutation of SRP14 alters its migration on SDS-PAGE without impacting its function.

ABSTRACT: SRP14 is a crucial protein subunit of the signal recognition particle (SRP), a ribonucleoprotein complex essential for co-translational translocation to the endoplasmic reticulum. During our investigation of SRP14 expression across diverse cell lines, we observe variations in its migration on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), with some cells exhibiting slower migration and others migrating faster. However, the cause of this phenomenon remains elusive. Our research rules out alternative splicing as the cause and, instead, identifies the presence of a P124A mutation in SRP14 (SRP14 ^P124A) among the faster-migrating variants, while the slower-migrating variants lack this mutation. Subsequent ectopic expression of wild-type SRP14 ^P124 o

SUBMITTER: Liu Y 

PROVIDER: S-EPMC10984872 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature