RNA m⁵C modification upregulates E2F1 expression in a manner dependent on YBX1 phase separation and promotes tumor progression in ovarian cancer.

ABSTRACT: 5-Methylcytosine (m⁵C) is a common RNA modification that modulates gene expression at the posttranscriptional level, but the crosstalk between m⁵C RNA modification and biomolecule condensation, as well as transcription factor-mediated transcriptional regulation, in ovarian cancer, is poorly understood. In this study, we revealed that the RNA methyltransferase NSUN2 facilitates mRNA m⁵C modification and forms a positive feedback regulatory loop with the transcription factor E2F1 in ovarian cancer. Specifically, NSUN2 promotes m⁵C modification of E2F1 mRNA and increases its stability, and E2F1 binds to the NSUN2 promoter, subsequently reciprocally activating NSUN2 transcription. The RNA binding protein YBX1 functions as the m⁵C reader an

SUBMITTER: Liu X 

PROVIDER: S-EPMC10984993 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature