Pooled CRISPR screening of high-content cellular phenotypes using ghost cytometry.

ABSTRACT: Recent advancements in image-based pooled CRISPR screening have facilitated the mapping of diverse genotype-phenotype associations within mammalian cells. However, the rapid enrichment of cells based on morphological information continues to pose a challenge, constraining the capacity for large-scale gene perturbation screening across diverse high-content cellular phenotypes. In this study, we demonstrate the applicability of multimodal ghost cytometry-based cell sorting, including both fluorescent and label-free high-content phenotypes, for rapid pooled CRISPR screening within vast cell populations. Using the high-content cell sorter operating in fluorescence mode, we successfully executed kinase-specific CRISPR screening targeting genes influencing the nuclear translocation of RelA. Furt

SUBMITTER: Tsubouchi A 

PROVIDER: S-EPMC10985231 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature