Synchrotron-source micro-x-ray computed tomography for examining butterfly eyes.

ABSTRACT: Comparative anatomy is an important tool for investigating evolutionary relationships among species, but the lack of scalable imaging tools and stains for rapidly mapping the microscale anatomies of related species poses a major impediment to using comparative anatomy approaches for identifying evolutionary adaptations. We describe a method using synchrotron source micro-x-ray computed tomography (syn-μXCT) combined with machine learning algorithms for high-throughput imaging of Lepidoptera (i.e., butterfly and moth) eyes. Our pipeline allows for imaging at rates of ~15 min/mm³ at 600 nm³ resolution. Image contrast is generated using standard electron microscopy labeling approaches (e.g., osmium tetroxide) that unbiasedly labels all cellular membranes in a species-ind

SUBMITTER: Paukner D 

PROVIDER: S-EPMC10985371 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature