Single-cell analysis of the CD8⁺ T-cell compartment in multiple myeloma reveals disease specific changes are chiefly restricted to a CD69^- subset suggesting potent cytotoxic effectors exist within the tumor bed.

ABSTRACT: Multiple myeloma (MM) is an incurable disease of the bone marrow (BM) characterized by the uncontrolled proliferation of neoplastic plasma cells. While CD8+ T cells have an established role in disease control, few studies have focused on these cells within the MM tumor microenvironment (TME). We analyzed CD8+ T cells in the BM and peripheral blood (PB) of untreated patients with MM and non-myeloma controls using flow cytometry, mass cytometry and single-cell RNA sequencing, using several novel bioinformatics workflows. Inter-tissue differences were most evident in the differential expression of Granzymes B and K, which were strongly associated with two distinct subsets of CD8+ T cells delineated by the expression of CD69, accounting for roughly 50% of BM-CD8+ T cells of all assessed cohort

SUBMITTER: Favaloro J 

PROVIDER: S-EPMC10985429 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature