LC-MS/DIA-based strategy for comprehensive flavonoid profiling: an Ocotea spp. applicability case.

ABSTRACT: We introduce a liquid chromatography - mass spectrometry with data-independent acquisition (LC-MS/DIA)-based strategy, specifically tailored to achieve comprehensive and reliable glycosylated flavonoid profiling. This approach facilitates in-depth and simultaneous exploration of all detected precursors and fragments during data processing, employing the widely-used open-source MZmine 3 software. It was applied to a dataset of six Ocotea plant species. This framework suggested 49 flavonoids potentially newly described for these plant species, alongside 45 known features within the genus. Flavonols kaempferol and quercetin, both exhibiting O-glycosylation patterns, were particularly prevalent. Gas-phase fragmentation reactions further supported these findings. For the first tim

SUBMITTER: Alves MF 

PROVIDER: S-EPMC10985591 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature