Testing region selection and prognostic analysis of MLH1 promoter methylation in colorectal cancer in China.

ABSTRACT:

Background

MLH1 promoter methylation analysis is recommended in screening for Lynch syndrome (LS) in patients with MLH1-deficient colorectal cancer (CRC). The study aims to identify specific methylation regions in the MLH1 promoter and to evaluate the clinicopathologic characteristics of and prognosis for patients with MLH1 methylation.

Methods

A total of 580 CRC cases were included. The DNA mismatch repair (MMR) protein expression was assessed by using immunohistochemistry (IHC). The methylation status of the Regions A, B, C, D, and E in the MLH1 promoter was tested by using bisulfite sequencing PCR. The specificities of the five regions were calculated. Associations between MLH1 methylation and clinicopathologic characteristics were evalu

SUBMITTER: Tan X 

PROVIDER: S-EPMC10985700 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature