Profiling the role of m6A effectors in the regulation of pluripotent reprogramming.

ABSTRACT: The N⁶-methyladenosine (m⁶A) RNA modification plays essential roles in multiple biological processes, including stem cell fate determination. To explore the role of the m6A modification in pluripotent reprogramming, we used RNA-seq to map m6A effectors in human iPSCs, fibroblasts, and H9 ESCs, as well as in mouse ESCs and fibroblasts. By integrating the human and mouse RNA-seq data, we found that 19 m6A effectors were significantly upregulated in reprogramming. Notably, IGF2BPs, particularly IGF2BP1, were among the most upregulated genes in pluripotent cells, while YTHDF3 had high levels of expression in fibroblasts. Using quantitative PCR and Western blot, we validated the pluripotency-associated elevation of IGF2BPs. Knockdown of IGF2BP1 induced the downregulation o

SUBMITTER: Wang W 

PROVIDER: S-EPMC10986062 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature