16S rRNA seq-identified Corynebacterium promotes pyroptosis to aggravate diabetic foot ulcer.

ABSTRACT:

Background

Diabetic foot ulcer (DFU) is one of the main chronic complications caused by diabetes, leading to amputation in severe cases. Bacterial infection affects the wound healing in DFU.

Methods

DFU patients who met the criteria were selected, and the clinical data were recorded in detail. The pus exudate from the patient's foot wound and venous blood were collected for biochemical analysis. The distribution of bacterial flora in pus exudates of patients was analyzed by 16S rRNA sequencing, and the correlation between DFU and pathogenic variables, pyroptosis and immunity was analyzed by statistical analysis. Then, the effects of key bacteria on the inflammation, proliferation, apoptosis, and pyroptosis of polymorphonuclear leukocytes were investigated by ELISA, CCK-8, fl

SUBMITTER: Zheng H 

PROVIDER: S-EPMC10986075 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature