Experimental and analytical pipeline for sub-genomic RNA landscape of coronavirus by Nanopore sequencer.

ABSTRACT: Coronaviruses (CoVs), including severe acute respiratory syndrome coronavirus 2, can infect a variety of mammalian and avian hosts with significant medical and economic consequences. During the life cycle of CoV, a coordinated series of subgenomic RNAs, including canonical subgenomic messenger RNA and non-canonical defective viral genomes (DVGs), are generated with different biological implications. Studies that adopted the Nanopore sequencer (ONT) to investigate the landscape and dynamics of viral RNA subgenomic transcriptomes applied arbitrary bioinformatics parameters without justification or experimental validation. The current study used bovine coronavirus (BCoV), which can be performed under biosafety level 2 for library construction and experimental validation using traditional colo

SUBMITTER: Chen B-J 

PROVIDER: S-EPMC10986531 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature