<i>Pseudomonas aeruginosa</i> transcriptome analysis of metal restriction in <i>ex vivo</i> cystic fibrosis sputum.

ABSTRACT: Chronic Pseudomonas aeruginosa lung infections are a feature of cystic fibrosis (CF) that many patients experience even with the advent of highly effective modulator therapies. Identifying factors that impact P. aeruginosa in the CF lung could yield novel strategies to eradicate infection or otherwise improve outcomes. To complement published P. aeruginosa studies using laboratory models or RNA isolated from sputum, we analyzed transcripts of strain PAO1 after incubation in sputum from different CF donors prior to RNA extraction. We compared PAO1 gene expression in this "spike-in" sputum model to that for P. aeruginosa grown in synthetic cystic fibrosis sputum medium to determine key genes, which are among the most differentially expressed or most highly express

SUBMITTER: Neff SL 

PROVIDER: S-EPMC10986534 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature