Approaches for identifying and measuring heteroresistance in azole-susceptible Candida isolates.

ABSTRACT: Heteroresistance to antifungal agents poses a significant challenge in the treatment of fungal infections. Currently, the absence of established methods for detecting and measuring heteroresistance impedes progress in understanding this phenomenon in fungal pathogens. In response to this gap, we present a comprehensive set of new and optimized methods designed to detect and quantify azole heteroresistance in Candida albicans. Here, we define two primary assays for measuring heteroresistance: population analysis profiling, based on growth on solid medium, and single-cell assays, based on growth in liquid culture. We observe good correlations between the measurements obtained with liquid and solid assays, validating their utility for studying azole heteroresistance. We also highlight

SUBMITTER: Gautier C 

PROVIDER: S-EPMC10986555 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature