Robust and sensitive amplicon-based whole-genome sequencing assay of respiratory syncytial virus subtype A and B.

ABSTRACT: Prevention of respiratory syncytial virus (RSV) infection is now a global health priority, with a long-acting monoclonal antibody and two RSV vaccines recently licenced for clinical use. Most licenced and candidate interventions target the RSV fusion (RSV-F) protein. New interventions may be associated with the spread of mutations, reducing susceptibility to antibody neutralization in RSV-F. There is a need for ongoing longitudinal global surveillance of circulating RSV strains. To achieve this large-scale genomic surveillance, a reliable, high-throughput RSV sequencing assay is required. Here we report an improved high-throughput RSV whole-genome sequencing (WGS) assay performed directly on clinical samples without additional enrichment, using a 4-primer-pool, short-amplicon PCR-tiling ap

SUBMITTER: Talts T 

PROVIDER: S-EPMC10986592 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature