In situ neutron scattering of antibody adsorption during protein A chromatography.

ABSTRACT: A deeper understanding of the nanoscale and mesoscale structure of chromatographic adsorbents and the distribution of proteins within the media, is critical to a mechanistic understanding of separation processes using these materials. Characterisation of the media's architecture at this scale and protein adsorption within, is challenging using conventional techniques. In this study, we propose a novel resin characterisation technique that enables in-situ measurement of the structure of the adsorbed protein layer within the resin, under typical chromatographic conditions. A quartz flow-through cell was designed and fabricated for use with Small Angle Neutron Scattering (SANS), in order to measure the nanoscale to mesoscale structures of a silica based protein A chromatography resin during t

SUBMITTER: Papachristodoulou M 

PROVIDER: S-EPMC10986645 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature