Spatiotemporally Precise Optical Manipulation of Intracellular Molecular Activities.

ABSTRACT: Controlling chemical processes in live cells is a challenging task. The spatial heterogeneity of biochemical reactions in cells is often overlooked by conventional means of incubating cells with desired chemicals. A comprehensive understanding of spatially diverse biochemical processes requires precise control over molecular activities at the subcellular level. Herein, a closed-loop optoelectronic control system is developed that allows the manipulation of biomolecular activities in live cells at high spatiotemporal precision. Chemical-selective fluorescence signals are utilized to command lasers that trigger specific chemical processes or control the activation of photoswitchable inhibitors at desired targets. This technology is fully compatible with laser scanning confocal fluorescence m

SUBMITTER: Dong B 

PROVIDER: S-EPMC10987104 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature