Exploring ex vivo biofilm dynamics: consequences of low ampicillin concentrations on the human oral microbiome.

ABSTRACT: Prolonged exposure to antibiotics at low concentration can promote processes associated with bacterial biofilm formation, virulence and antibiotic resistance. This can be of high relevance in microbial communities like the oral microbiome, where commensals and pathogens share a common habitat and where the total abundance of antibiotic resistance genes surpasses the abundance in the gut. Here, we used an ex vivo model of human oral biofilms to investigate the impact of ampicillin on biofilm viability. The ecological impact on the microbiome and resistome was investigated using shotgun metagenomics. The results showed that low concentrations promoted significant shifts in microbial taxonomic profile and could enhance biofilm viability by up to 1 to 2-log. For the resistome, low concentratio

SUBMITTER: Brar NK 

PROVIDER: S-EPMC10987642 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature