Rewiring of the phosphoproteome executes two meiotic divisions in budding yeast.

ABSTRACT: The cell cycle is ordered by a controlled network of kinases and phosphatases. To generate gametes via meiosis, two distinct and sequential chromosome segregation events occur without an intervening S phase. How canonical cell cycle controls are modified for meiosis is not well understood. Here, using highly synchronous budding yeast populations, we reveal how the global proteome and phosphoproteome change during the meiotic divisions. While protein abundance changes are limited to key cell cycle regulators, dynamic phosphorylation changes are pervasive. Our data indicate that two waves of cyclin-dependent kinase (Cdc28^Cdk1) and Polo (Cdc5^Polo) kinase activity drive successive meiotic divisions. These two distinct phases of phosphorylation are ensured by the meiosis-s

SUBMITTER: Koch LB 

PROVIDER: S-EPMC10987667 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature