Global Interactome Mapping Reveals Pro-tumorigenic Interactions of NF-κB in Breast Cancer.

ABSTRACT: NF-κB pathway is involved in inflammation; however, recent data shows its role also in cancer development and progression, including metastasis. To understand the role of NF-κB interactome dynamics in cancer, we study the complexity of breast cancer interactome in luminal A breast cancer model and its rearrangement associated with NF-κB modulation. Liquid chromatography-mass spectrometry measurement of 160 size-exclusion chromatography fractions identifies 5460 protein groups. Seven thousand five hundred sixty eight interactions among these proteins have been reconstructed by PrInCE algorithm, of which 2564 have been validated in independent datasets. NF-κB modulation leads to rearrangement of protein complexes involved in NF-κB signaling and immune response, cell cycle regulation, and DNA

SUBMITTER: Lapcik P 

PROVIDER: S-EPMC10988130 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature