Mesenchymal stem cells reverse thymus aging by reprogramming the DNA methylation of thymic epithelial cells.

ABSTRACT:

Background

A decrease in the number and activity of thymic epithelial cells (TECs) is an important factor in thymic degeneration. Mesenchymal stem cells (MSCs) treating thymic ageing is a promising strategy, but the DNA methylation modification mechanism in TECs remains unclear.

Methods

Aged rhesus monkeys were treated with MSCs to establish a thymic senescence model, and hematoxylin-eosin (HE) staining, immunofluorescence staining, and ELISA were performed to observe the structure and function of the thymus. TEC aging model and MSCs co-culture system were established to detect DNA methylation modification and transcriptomic changes, correlation analysis between transcription factor methylation and mRNA expression, and q-PCR, immunofluorescence staining, and Western blot wer

SUBMITTER: Yang Z 

PROVIDER: S-EPMC10988135 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature