Biogenic Zinc Oxide Nanoparticles synthesized from <i>Tinospora Cordifolia</i> induce oxidative stress, mitochondrial damage and apoptosis in Colorectal Cancer.

ABSTRACT: Cancer chemotherapy remains a serious challenge, and new approaches to therapy are urgently needed to build novel treatment regimens. The methanol extract of the stem of Tinospora Cordifolia was used to synthesize biogenic zinc oxide nanoparticles (ZnO-NPs) that display anticancer activities against colorectal cancer. Biogenic ZnO-NPs synthesized from methanol extract of Tinospora Cordifolia stem (ZnO-NPs TM) were tested against HCT-116 cell lines to assess anticancer activity. UV-Vis, FTIR, XRD, SEM, and TEM analysis characterized the biogenic ZnO-NPs. To see how well biogenic ZnO-NPs fight cancer, cytotoxicity, AO/EtBr staining, Annexin V/PI staining, mitochondrial membrane potential (MMP), generation of reactive oxygen species (ROS) analysis, and caspase cascade activity a

SUBMITTER: Berehu HM 

PROVIDER: S-EPMC10988208 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature