Identification of apolipoprotein B-reactive CDR3 motifs allows tracking of atherosclerosis-related memory CD4⁺T cells in multiple donors.

ABSTRACT:

Introduction

Atherosclerosis is a major pathological condition that underlies many cardiovascular diseases (CVDs). Its etiology involves breach of tolerance to self, leading to clonal expansion of autoreactive apolipoprotein B (APOB)-reactive CD4⁺T cells that correlates with clinical CVD. The T-cell receptor (TCR) sequences that mediate activation of APOB-specific CD4⁺T cells are unknown.

Methods

In a previous study, we had profiled the hypervariable complementarity determining region 3 (CDR3) of CD4⁺T cells that respond to six immunodominant APOB epitopes in most donors. Here, we comprehensively analyze this dataset of 149,065 APOB-reactive and 199,211 non-reactive control CDR3s from six human leukocyte antigen-typed donors.

Results

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SUBMITTER: Roy P 

PROVIDER: S-EPMC10988780 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature