Simultaneous targeted and discovery-driven clinical proteotyping using hybrid-PRM/DIA.

ABSTRACT:

Background

Clinical samples are irreplaceable, and their transformation into searchable and reusable digital biobanks is critical for conducting statistically empowered retrospective and integrative research studies. Currently, mainly data-independent acquisition strategies are employed to digitize clinical sample cohorts comprehensively. However, the sensitivity of DIA is limited, which is why selected marker candidates are often additionally measured targeted by parallel reaction monitoring.

Methods

Here, we applied the recently co-developed hybrid-PRM/DIA technology as a new intelligent data acquisition strategy that allows for the comprehensive digitization of rare clinical samples at the proteotype level. Hybrid-PRM/DIA enables enhanced measurement sensitivity for a spe

SUBMITTER: Goetze S 

PROVIDER: S-EPMC10988896 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature