Antibiotic-induced ROS-mediated Fur allosterism contributes to <i>Helicobacter pylori</i> resistance by inhibiting <i>arsR</i> activation of <i>mutS</i> and <i>mutY</i>.

ABSTRACT: The increasing antibiotic resistance of Helicobacter pylori primarily driven by genetic mutations poses a significant clinical challenge. Although previous research has suggested that antibiotics could induce genetic mutations in H. pylori, the molecular mechanisms regulating the antibiotic induction remain unclear. In this study, we applied various techniques (e.g., fluorescence microscopy, flow cytometry, and multifunctional microplate reader) to discover that three different types of antibiotics could induce the intracellular generation of reactive oxygen species (ROS) in H. pylori. It is well known that ROS, a critical factor contributing to bacterial drug resistance, not only induces damage to bacterial genomic DNA but also inhibits the expression of genes associa

SUBMITTER: Xue J 

PROVIDER: S-EPMC10989006 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature