Expression of HIF‑α and their association with clinicopathological parameters in clinical renal cell carcinoma.

ABSTRACT:

Objectives

This study aimed to assess the cellular localization and expression levels of hypoxia-inducible factor (HIF) -α proteins (specifically HIF-1α, HIF-2α, and HIF-3α) that play a role in the hypoxia pathway and to determine their correlation with clinicopathological parameters and patient survival in renal cell carcinoma (RCC).

Materials and methods

Tissue microarray (TMA) with cores from 150 clear cell RCCs and 31 non-ccRCC samples. HIF-1α, HIF-2α, and HIF-3α antibodies were used for immunohistochemistry (IHC) of TMA to evaluate the cellular localization and expression levels of HIF-α proteins, specifically in relation to the hypoxia pathway.

Results

The expression levels of the HIF-α proteins were higher in the nucleus than in the cytoplasm. Furthermore, the

SUBMITTER: Sitaram RT 

PROVIDER: S-EPMC10989218 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature