Isolation, separation, identification, and quantification of bioactive methylated flavone regioisomers by UHPLC-MS/MS.

ABSTRACT: Methylated flavones, commonly found in many plants of the Brassicaceae family, have potent antioxidant and anticancer activity with diverse therapeutic potential. However, the specific regioisomers of methylated flavones can have significantly different biochemical and potentially therapeutic properties as shown by various bioassays but analytically differentiating these compounds has been technically challenging and rarely reported. In this study, we demonstrate differentiation and identification of selected bioactive methylated flavone regioisomers, namely 5,7,3'-trihydroxy-4'-methoxyflavone, and 5,7,4'-trihydroxy-3'-methoxyflavone extracted from Coronopus didymus, a member of the Brassicaceae family, using ultra-performance liquid chromatography coupled with electrospray ionizati

SUBMITTER: Noreen H 

PROVIDER: S-EPMC10989521 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature