A global metabolomics minefield: Confounding effects of preanalytical factors when studying rare disorders.

ABSTRACT: A common challenge when studying rare diseases or medical conditions is the limited number of patients, usually resulting in long inclusion periods as well as unequal sampling and storage conditions. The main purpose of this study was to demonstrate the challenges when comparing samples subject to different preanalytical conditions. We performed a global (commonly referred to as "untargeted") liquid chromatography-high resolution mass spectrometry metabolomics analysis of blood samples from cases of sudden infant death syndrome and controls stored as dried blood spots on a chemical-free filter card for 15 years at room temperature compared with the same blood samples stored as whole blood at -80°C before preparing new dried blood spots using a chemically treated filter card. Principal comp

SUBMITTER: Skogvold HB 

PROVIDER: S-EPMC10989595 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature