Single-cell Raman and functional gene analyses reveal microbial P solubilization in agriculture waste-modified soils.

ABSTRACT: Application of agricultural waste such as rapeseed meal (RM) is regarded as a sustainable way to improve soil phosphorus (P) availability by direct nutrient supply and stimulation of native phosphate-solubilizing microorganisms (PSMs) in soils. However, exploration of the in situ microbial P solubilizing function in soils remains a challenge. Here, by applying both phenotype-based single-cell Raman with D₂O labeling (Raman-D₂O) and genotype-based high-throughput chips targeting carbon, nitrogen and P (CNP) functional genes, the effect of RM application on microbial P solubilization in three typical farmland soils was investigated. The abundances of PSMs increased in two alkaline soils after RM application identified by single-cell Raman D₂O. RM application

SUBMITTER: Li H 

PROVIDER: S-EPMC10989763 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature