High-efficiency genome editing of an extreme thermophile <i>Thermus thermophilus</i> using endogenous type I and type III CRISPR-Cas systems.

ABSTRACT: Thermus thermophilus is an attractive species in the bioindustry due to its valuable natural products, abundant thermophilic enzymes, and promising fermentation capacities. However, efficient and versatile genome editing tools are not available for this species. In this study, we developed an efficient genome editing tool for T. thermophilus HB27 based on its endogenous type I-B, I-C, and III-A/B CRISPR-Cas systems. First, we systematically characterized the DNA interference capabilities of the different types of the native CRISPR-Cas systems in T. thermophilus HB27. We found that genomic manipulations such as gene deletion, mutation, and in situ tagging could be easily implemented by a series of genome-editing plasmids carrying an artificial self-targeting mini-CRISPR

SUBMITTER: Wang J 

PROVIDER: S-EPMC10989782 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature