A k-mer-based bulked segregant analysis approach to map seed traits in unphased heterozygous potato genomes.

ABSTRACT: High-throughput sequencing-based methods for bulked segregant analysis (BSA) allow for the rapid identification of genetic markers associated with traits of interest. BSA studies have successfully identified qualitative (binary) and quantitative trait loci (QTLs) using QTL mapping. However, most require population structures that fit the models available and a reference genome. Instead, high-throughput short-read sequencing can be combined with BSA of k-mers (BSA-k-mer) to map traits that appear refractory to standard approaches. This method can be applied to any organism and is particularly useful for species with genomes diverged from the closest sequenced genome. It is also instrumental when dealing with highly heterozygous and potentially polyploid genomes without phased haplotype asse

SUBMITTER: Sonsungsan P 

PROVIDER: S-EPMC10989861 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature