Plasma ctDNA liquid biopsy of IDH1, TERTp, and EGFRvIII mutations in glioma.

ABSTRACT:

Background

Circulating tumor DNA has emerging clinical applications in several cancers; however, previous studies have shown low sensitivity in glioma. We investigated if 3 key glioma gene mutations IDH1, TERTp, and EGFRvIII could be reliably detected in plasma by droplet digital polymerase chain reaction (ddPCR) thereby demonstrating the potential of this technique for glioma liquid biopsy.

Methods

We analyzed 110 glioma patients from our biobank with a total of 359 plasma samples (median 4 samples per patient). DNA was isolated from plasma and analyzed for IDH1, TERTp, and EGFRvIII mutations using ddPCR.

Results

Total cfDNA was significantly associated with tumor grade, tumor volume, and both overall and progression-free sur

SUBMITTER: Jones JJ 

PROVIDER: S-EPMC10989869 | biostudies-literature | 2024 Jan-Dec

REPOSITORIES: biostudies-literature