Luminal transport through intact endoplasmic reticulum limits the magnitude of localized Ca²⁺ signals.

ABSTRACT: The endoplasmic reticulum (ER) forms an interconnected network of tubules stretching throughout the cell. Understanding how ER functionality relies on its structural organization is crucial for elucidating cellular vulnerability to ER perturbations, which have been implicated in several neuronal pathologies. One of the key functions of the ER is enabling Ca[Formula: see text] signaling by storing large quantities of this ion and releasing it into the cytoplasm in a spatiotemporally controlled manner. Through a combination of physical modeling and live-cell imaging, we demonstrate that alterations in ER shape significantly impact its ability to support efficient local Ca[Formula: see text] releases, due to hindered transport of luminal content within the ER. Our model reveals that rapid Ca[

SUBMITTER: Crapart CC 

PROVIDER: S-EPMC10990089 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature