Dual topologies of myotomal collagen XV and Tenascin C act in concert to guide and shape developing motor axons.

ABSTRACT: During development, motor axons are guided toward muscle target by various extrinsic cues including extracellular matrix (ECM) proteins whose identities and cellular source remain poorly characterized. Here, using single-cell RNAseq of sorted GFP⁺ cells from smyhc1:gfp-injected zebrafish embryos, we unravel the slow muscle progenitors (SMP) pseudotemporal trajectory at the single-cell level and show that differentiating SMPs are a major source of ECM proteins. The SMP core-matrisome was characterized and computationally predicted to form a basement membrane-like structure tailored for motor axon guidance, including basement membrane-associated ECM proteins, as collagen XV-B, one of the earliest core-matrisome gene transcribed in differentiating SMPs and the glycoprotein T

SUBMITTER: Nemoz-Billet L 

PROVIDER: S-EPMC10990108 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature