Reconstitution of a biofilm adhesin system from a sulfate-reducing bacterium in <i>Pseudomonas fluorescens</i>.

ABSTRACT: Biofilms of sulfate-reducing bacterium (SRB) like Desulfovibrio vulgaris Hildenborough (DvH) can facilitate metal corrosion in various industrial and environmental settings leading to substantial economic losses. Although the mechanisms of biofilm formation by DvH are not yet well understood, recent studies indicate the large adhesin, DvhA, is a key determinant of biofilm formation. The dvhA gene neighborhood resembles the biofilm-regulating Lap system of Pseudomonas fluorescens but is curiously missing the c-di-GMP-binding regulator LapD. Instead, DvH encodes an evolutionarily unrelated c-di-GMP-binding protein (DVU1020) that we hypothesized is functionally analogous to LapD. To study this unusual Lap system and overcome experimental limitations with the slow-growing

SUBMITTER: Karbelkar AA 

PROVIDER: S-EPMC10990149 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature