EndoGeneAnalyzer: A tool for selection and validation of reference genes.

ABSTRACT: The selection of proper reference genes is critical for accurate gene expression analysis in all fields of biological and medical research, mainly because there are many distinctions between different tissues and specimens. Given this variability, even in known classic reference genes, demands of a comprehensive analysis platform is needed to identify the most suitable genes for each study. For this purpose, we present an analysis tool for assisting in decision-making in the analysis of reverse transcription-quantitative polymerase chain reaction (RT-qPCR) data. EndoGeneAnalyzer, an open-source web tool for reference gene analysis in RT-qPCR studies, was used to compare the groups/conditions under investigation. This interactive application offers an easy-to-use interface that allows effic

SUBMITTER: Teixeira EB 

PROVIDER: S-EPMC10990236 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature